Aldohexose to Ketohexose Transformation
The conversion of an aldose (like D-Glucose) to an isomeric ketose (like D-Fructose) is performed through the formation of an osazone intermediate.
The Osazone Method Mechanism
- Osazone Formation: The aldose reacts with an excess of phenylhydrazine to form the corresponding osazone.
- Hydrolysis: The osazone is hydrolyzed using dilute HCl to produce an osone.
- Reduction: The osone is selectively reduced using Zinc (Zn) and glacial acetic acid to yield the corresponding isomeric ketose.