Conversion of Arabinose to Glucose and Mannose

Kiliani-Fischer Cyanohydrin Synthesis

The Kiliani-Fischer synthesis is used to increase the carbon chain length of an aldose by one carbon atom. The process converts an aldopentose (like D-Arabinose) into a mixture of two epimeric aldohexoses (D-Glucose and D-Mannose).

Mechanism Steps

  1. Cyanohydrin Formation: The aldose reacts with HCN, creating a new asymmetric center and producing two isomeric cyanohydrins.
  2. Hydrolysis: The cyanohydrins are hydrolyzed with dilute acid to form corresponding aldonic acids.
  3. Lactonization: Heating the aldonic acids results in the loss of water, forming γ-lactones.
  4. Reduction: The lactones are reduced using Lithium Aluminum Hydride (LAH) or Na-Hg in a weakly acidic solution to yield the final aldoses.
Chemical mechanism of Kiliani-Fischer synthesis showing conversion of Arabinose to Glucose and Mannose
Conversion of D-Arabinose to D-Glucose and D-Mannose.

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